Phosphoinositide Binding Specificity among Phospholipase C Isozymes as Determined by Photo-Cross-Linking to Novel Substrate and Product Analogs
Biochemistry, 36(23), 7239- 7724, June 1999
Edward G. Tall, Ph.D. Department of Biological Studies
G. Dorman, P. Garcia, L. Runnels, S. Shah, J. Chen, A. Profit, Q. Gu, A. Chaudhary, G.D. Prestwich & M.J. Rebecchi
The results demonstrate that the PH domain is the sole high-affinity PI(4,5)P2 binding site of PLC-delta1 and that a similar site is not present in PLC-beta1, -beta2, or -beta3. The data are consistent with the idea that the PH domain of PLC-delta1, but not the beta isozymes, directs the catalytic core to membranes enriched in PI(4,5)P2 and is subject to product inhibition.